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技術(shù)文章您現(xiàn)在的位置：首頁 > 技術(shù)文章 > 在枯草芽孢桿菌中表達B.cereus ATCC14579的異源核黃素操縱子提高核黃素產(chǎn)量

在枯草芽孢桿菌中表達B.cereus ATCC14579的異源核黃素操縱子提高核黃素產(chǎn)量

更新時間：2013-07-26   點擊次數(shù)：2728次

Fragment containing the whole riboflavin（rib）operons of B.cereus ATCC14579 was detected from GenBank and annotated.The rib operon of ATCC14579 was cloned with Pn,its native promoter,or with P43,the vegetative growth promoter,into the plasmid.Expression analysis showed that heterologous rib operon was operative in B.subtilis.Integrative plasmid with P43-rib fragment was integrated into the chromosome of B.subtilis RH33,yielding transformant B.subtilis PY.With optimized medium components

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